Development of Breeder-Friendly KASP Markers for Low Concentration of Kunitz Trypsin Inhibitor in Soybean Seeds
نویسندگان
چکیده
منابع مشابه
Isolation and Characterization of Trypsin Inhibitors (Kunitz Soybean Trypsin Inhibitor, Bowman-birk Inhibitor) in Soybean
Soybean (Glycine max (L.) Merr.) has emerged as one of the most economical and nutritious foods. Kunitz Soybean Trypsin Inhibitor (KSTI) and Bowman -Birk Inhibitor (BBI) are the two major trypsin inhibitors in soybeans. The ingested soybean trypsin inhibitors cause growth depression as demonstrated in different animal species and human. The main part of the present study was devoted to isolatio...
متن کاملStability of the allergenic soybean Kunitz trypsin inhibitor.
The soybean Kunitz trypsin inhibitor (SKTI) is a 21.5 kDa allergenic protein that belongs to the family of all antiparallel beta-sheet proteins that are highly resistant to thermal and chemical denaturation. Spectroscopic and biochemical techniques such as circular dichroism (CD), ANS fluorescence and proteolysis were used to study its molecular structure under denaturing conditions such as aci...
متن کاملDeployment of gene specific marker in development of kunitz trypsin inhibitor free soybean genotypes.
Genetic elimination of kunitz trypsin inhibitor in soybean seed would obviate the need for boiling required to inactivate the antinutritional factor and therefore economize the soy processing. PI542044, the source of null variant of kunitz trypsin inhibitor gene is being used in the development of kunitz trypsin inhibitor free soybean genotypes in India. Gene specific marker can expedite the ge...
متن کاملMolecular and Biochemical Characterization of Soybean Genotypes for the Presence of Kunitz Trypsin Inhibitor
Present study was conducted to characterize soybean genotypes for the kunitz trypsin inhibitor at molecular and biochemical level. Genotypes used in the present study included one null KTI soybean line PI542044 (the source of null allele of kunitz trypsin inhibitor being used in soybean breeding programs in India), DSRtiti (homozygous recessive genotype for KTi gene) and seven other elite soybe...
متن کاملMarker assisted accelerated introgression of null allele of kunitz trypsin inhibitor in soybean
Development of kunitz trypsin inhibitor (KTI)-free soybean is crucial for soy-food industry as the heat inactivation employed to inactivate the anti-nutritional factor in regular soybean incurs extra cost and affects protein solubility. In the presented work, a null allele of KTI from PI542044 was introgressed into cultivar 'JS97-52' (recurrent parent) through marker assisted backcrossing. Fore...
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2021
ISSN: 1422-0067
DOI: 10.3390/ijms22052675